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对固有免疫信号分子hsa-miR-181a进行生物信息学分析,以期探讨其调节脑卒中的生物学途径和信号通路,为hsamiR-181a的实验验证及功能研究提供理论指导。利用TargetScan 6.0、miRWalk、miRanda、PicTar、Genecards数据库预测所得的hsa-miR-181a与脑卒中所相关的靶基因,通过BINGO及DAVID对脑卒中相关靶基因集合进行基因本体(gene ontology,GO)和信号通路分析。hsa-miR-181a可与固有免疫基因TLR4相互作用,并同时作用于与脑卒中相关的其它靶基因,通过调节细胞途径,影响代谢过程等生物学途径,神经营养因子信号转导等KEGG信号通路发挥作用。固有免疫信号分子hsa-miR-181a可通过调节细胞途径,影响代谢过程等生物学途径,神经营养因子信号转导等信号通路调节脑卒中,为进一步实验和功能验证提供生物信息学指导。
The bioinformatics analysis of hsa-miR-181a, an innate immune signaling molecule, was carried out to investigate the biological pathways and signal pathways that regulate stroke, providing theoretical guidance for the experimental verification and functional study of hsamiR-181a. The target genes related to stroke with hsa-miR-181a were predicted by using TargetScan 6.0, miRWalk, miRanda, PicTar and Genecards databases. Genes of stroke-related target genes were genotyped by BINGO and DAVID gene ontology (GO) and Signal path analysis. hsa-miR-181a can interact with the innate immune gene TLR4 and simultaneously act on other target genes associated with stroke through regulating cellular pathways, affecting biological pathways such as metabolic processes, and signaling pathways such as neurotrophin signaling Play a role. The innate immune signaling molecule hsa-miR-181a can regulate the stroke by regulating the cellular pathways, affecting biological pathways such as metabolic processes, and signaling pathways such as neurotrophin signaling, providing bioinformatics guidance for further experimental and functional validation.