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目的通过给新生大鼠吸入80%高浓度氧制作新生大鼠高氧脑损伤模型,并对其进行评价。方法新生Wistar大鼠72只,随机分为80%高氧模型组和空气对照组各36只,两组新生鼠分别于开始吸氧后24、48、72、96、120、144 h各处死6只,监测大鼠体重和脑重变化,并观察脑组织的形态学改变和神经元凋亡情况。结果两组新生Wistar大鼠入组时体重差异无统计学意义,模型组新生大鼠吸氧24 h后开始出现精神萎靡、少动、48~72 h出现对高氧的依赖,体重增长缓慢,吸氧5天后体重增长明显低于空气对照组,差异有统计学意义。模型组脑重总体较空气对照组轻,但差异无统计学意义,模型组吸氧48 h后脑组织切片示大脑皮质和海马区神经元轻度水肿,细胞核部分固缩、深染,神经元凋亡增多,随着吸氧时间的延长,细胞变性逐渐增多,5天达到高峰,神经元排列紊乱,部分神经元缺失,大量的神经凋亡细胞产生。结论 80%高浓度氧暴露120 h能够成功复制典型的新生大鼠高氧脑损伤模型。
OBJECTIVE: To establish neonatal rat models of hyperoxic brain injury by inhalation of 80% oxygen in neonatal rats. Methods Seventy two newborn Wistar rats were randomly divided into three groups: normoxia model group (80%) and air control group (n = 36). The newborn rats were sacrificed at 24,48,72,96,120,144 h Only rats, body weight and brain weight were monitored, and morphological changes of brain tissue and neuronal apoptosis were observed. Results There was no significant difference in body weight between the two groups of newborn Wistar rats. The newborn rats in model group began to appear apathetic and sedentary 24 hours after oxygen inhalation, and relied on hyperoxia 48 to 72 hours after hypoxia. The weight gain was slow, Body weight gain after 5 days of oxygen exposure was significantly lower than that of air control group, the difference was statistically significant. The brain weight of the model group was lighter than that of the air control group, but the difference was not statistically significant. In the model group, the neurons of the cerebral cortex and hippocampus were mildly edematous after 48 hours of oxygen inhalation. The pyknosis, With the increase of oxygen inhalation time, cell degeneration gradually increased, peaked at 5 days, neurons were disordered, some neurons were missing, and a large number of apoptotic neurons were produced. Conclusion A typical neonatal rat model of hyperoxic brain injury can be successfully replicated after being exposed to 80% oxygen for 120 h.