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目的本研究旨在探讨β-TCP(β-磷酸三钙)/P-15(P-15多肽)复合材料修复兔下颌骨骨缺损的能力。方法将β-TCP完全浸入到P-15多肽溶液中,制备出β-TCP/P-15多孔复合材料。在日本大耳白兔的下颌骨体部制备出骨缺损区,随机植入β-TCP/P-15(TP组)、β-TCP(T组)及空白对照组,在术后第4周、8周和12周分别处死动物,进行大体观察、组织学染色观察及成骨量(新生骨面积)的测定。结果随着时间的延长,三组骨缺损区的成骨量均增加,同一时间的不同组之间,以及同一组的不同时间段之间均有显著差异(P<0.001),TP组成骨量增加最快;各时间点TP组的成骨量均大于同一时间的对照组(P<0.001)。结论本实验中所用的β-TCP/P-15复合材料具有良好的骨诱导活性、降解性、生物相容性以及成骨性能,可促进骨缺损的修复,具有进一步的研究和临床应用价值。
Objective The purpose of this study was to investigate the ability of β-TCP (β-tricalcium phosphate) / P-15 (P-15 polypeptide) composite to repair rabbit mandibular defects. Methods β-TCP was completely immersed in P-15 peptide solution to prepare β-TCP / P-15 porous composite. The bone defect area was prepared in the mandibular body of Japanese white rabbits. The rats were randomly divided into three groups: β-TCP / P-15 group (TP group), β-TCP group (T group) and blank control group Animals were sacrificed at 8 weeks and 12 weeks respectively for gross observation, histological staining and bone formation (new bone area). Results With the prolongation of time, the osteogenic volume increased in all three groups, with significant difference (P <0.001) between different groups at the same time and in different time periods of the same group (P <0.001). The osteogenesis of TP group was higher than that of control group at the same time (P <0.001). Conclusion The β-TCP / P-15 composite used in this study has good osteoinductive activity, biodegradability, biocompatibility and osteogenic properties, which can promote the repair of bone defects. It has further research and clinical application value.