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目的 :①建立Ｔ细胞受体 (ＴＣＲ)δ、γ基因重排双标记的竞争性聚合酶链反应 (ＣＰＣＲ)—ＤＮＡ定量方法 ,并对儿童急性淋巴细胞白血病 (ＡＬＬ)化疗效应差异进行动态定量评价。②为确定ＰＣＲ产物中ＡＬＬ恶性细胞克隆的存在、印证ＣＰＣＲ方法的特异性 ,进行了ＰＣＲ产物Ｔ
OBJECTIVE: To establish a competitive polymerase chain reaction (CPCR) -DNA quantitative method for dual labeling of T cell receptor (TCR) δ and γ genes, and to dynamically quantify differences in chemotherapeutic effects in children with acute lymphoblastic leukemia (ALL) Evaluation. ② To determine the existence of ALL malignant cell clones in PCR products and verify the specificity of the CPCR method, PCR products T