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目的评价用卵蛋白(OVA)叠加高浓度臭氧(O3)暴露方法建立大鼠严重支气管哮喘模型的方法。方法随机数表法将30只SD大鼠分为正常组、卵蛋白激发组(OVA组)及卵蛋白叠加臭氧暴露组(OVA/O3)。采用OVA腹腔注射致敏,结合OVA持续雾化吸入和臭氧暴露方法,建立重度哮喘模型。观察大鼠哮喘发作期间症状,苏木素-伊红(HE)染色观察大鼠肺组织形态变化及炎症细胞浸润情况。采用免疫组化方法检测NF-κB p65亚基表达变化。结果以OVA激发期间OVA/O3大鼠出现最严重哮喘症状;HE染色结果显示OVA组及OVA/O3组有明显嗜酸性粒细胞浸润,并伴有支气管上皮细胞脱落,OVA/O3现象最为明显。p65在肺组织中的表达以平均光密度(IOD mean)计分别为:正常组(0.0146±0.0013),OVA组(0.2502±0.0009),OVA/O3(0.4240±0.0011),两模型组显著高于正常组,差异有统计学意义(P<0.01),OVA/O3组亦显著高于OVA组(P<0.01)。结论用臭氧暴露结合OVA致敏方法成功建立重度哮喘大鼠模型。
Objective To evaluate the method of establishing a rat model of severe bronchial asthma by overexposing ovalbumin (OVA) with high concentration ozone (O3) exposure. Methods 30 SD rats were divided into normal group, OVA group and ovalbumin overexposure group (OVA / O3). OVA intraperitoneal injection sensitization, combined with OVA continuous inhalation and ozone exposure methods, the establishment of severe asthma model. The symptoms of rats during asthma attack were observed. The lung tissue morphology and inflammatory cell infiltration were observed by hematoxylin-eosin (HE) staining. The expression of NF-κB p65 subunit was detected by immunohistochemistry. Results The OVA / O3 rats developed the most severe asthma symptoms during OVA challenge. HE staining showed that OVA and OVA / O3 groups had obvious eosinophilic infiltration accompanied by the shedding of bronchial epithelial cells with the most obvious OVA / O3 phenomenon. The expression of p65 in lung tissue was respectively (0.0146 ± 0.0013), OVA (0.2502 ± 0.0009) and OVA / O3 (0.4240 ± 0.0011) in IOD mean, which were significantly higher than those in model group In normal group, the difference was statistically significant (P <0.01), OVA / O3 group was also significantly higher than OVA group (P <0.01). Conclusion Overexpression of ozone combined with OVA sensitization successfully established a rat model of severe asthma.