目的:探讨囊胚形成的相关影响因素。方法:将IVF/ICSI移植后剩余的非优质胚胎行囊胚培养观察,分析患者年龄、胚胎数、本周期中优胚占总胚胎的比例以及培养环境等对囊胚形成的影响。结果:337例共2 168个胚胎进行囊胚培养,283例形成囊胚,形成囊胚3CC以上的860个,囊胚形成率为39.7%。移植周期中有囊胚形成组比无囊胚形成组的临床妊娠率高,三气培养(6%CO2、5%O2、89%N2)的囊胚形成率明显高于常规的两气培养(6%CO2、20%O2)。结论:对移植剩余的非优质胚胎可进行囊胚培养来对胚胎进行取舍,既保护了有发育潜能的胚胎又提高了胚胎的利用率。影响囊胚形成的因素主要有患者的年龄、卵子质量、卵裂期胚胎的质量、培养基及培养环境等。 Objective: To investigate the related factors of blastocyst formation. Methods: The remaining non-quality embryos after IVF / ICSI transplantation were observed in blastocysts culture. The age and number of embryos, the ratio of the total number of embryos in the cycle, the culture environment and so on were analyzed. Results: A total of 2 168 embryos from 337 cases were cultured in blastocysts, 283 blastocysts were formed, 860 blastocysts were formed, and the blastocyst formation rate was 39.7%. The pregnancy rate of blastocyst-forming group was higher than that of non-blastocyst-forming group in transplanted period. The blastocyst formation rate in three-gas culture (6% CO2, 5% O2, 89% N2) 6% CO2, 20% O2). Conclusion: The remaining non-quality embryos can be cultured in blastocysts to choose embryos, which not only protect the embryos with developmental potential but also improve the embryo utilization rate. The main factors affecting the formation of blastocysts age of patients, egg quality, the quality of cleavage embryos, culture medium and culture environment.