【摘 要】
：
Dear Editor,rnThe most advanced prime editor 3 (PE3) system comprises the editor,a fusion protein of Cas9 H840A nickase and mutant reverse transcriptase (RTase) (hereafter termed NMRT),a prime editing guide RNA (pegRNA) and an alternative single-guide RNA
【机 构】
：
Key Laboratory of Animal Genetics,Breeding and Reproduction of Shaanxi Province,College of Animal Sc
论文部分内容阅读
Dear Editor,rnThe most advanced prime editor 3 (PE3) system comprises the editor,a fusion protein of Cas9 H840A nickase and mutant reverse transcriptase (RTase) (hereafter termed NMRT),a prime editing guide RNA (pegRNA) and an alternative single-guide RNA (sgRNA).1 The pegRNA contains a primer binding site (PBS) and a reverse transcription (RT) template for introducing new genetic informa-tion1 (Fig.1a;Supplementary information,Fig.S1a).We noted that the PBS,which is generally 10-16 nt at the 3\'end of pegRNA,is complementary to part of the spacer at the 5\'end of pegRNA,and their annealing is expected to cause pegRNA circularization,which can potentially hamper editing (Fig.1 a;Supplementary information,Fig.S1 b,c).
其他文献
Whereas the critical roles of innate lymphoid cells (ILCs) in adult are increasingly appreciated,their developmental hierarchy in early human fetus remains largely elusive.In this study,we sorted human hematopoietic stem/progenitor cells,lymphoid progenit
Nucleic acid-based systems play important roles in antiviral defense,including CRISPR/Cas that adopts RNA-guided DNA cleavage to prevent DNA phage infection and RNA interference(RNAi)that employs RNA-guided RNA cleavage to defend against RNA virus infecti
Dear Editor,rnSARS-CoV-2 variants are developing rapidly among COVID-19 patients,likely resulting in higher transmissibility at the population level.1-5 Mutations in the spike proteins (S proteins) of these variants are supposed to be related with recepto
Pyroptosis is a form of regulated cell death mediated by gasdermin family members,among which the function of GSDMC has not been clearly described.Herein,we demonstrate that the metabolite α-ketoglutarate(α-KG)induces pyroptosis through caspase-8-mediated
Glioblastoma (GBM) is a prevalent and highly lethal form of glioma,with rapid tumor progression and frequent recurrence.Excessive outgrowth of pericytes in GBM governs the ecology of the perivascular niche,but their function in mediating chemoresistance h
Dear Editor,rnSevere acute respiratory syndrome coronavirus-2 (SARS-CoV-2),the causative agent of coronavirus disease 2019 (COVID-19),emerged in late 2019 and has since caused a pandemic.Although there have been extensive studies worldwide,our understandi
Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has caused a global crisis,urgently necessitating the development of safe,efficacious,convenient-to-store,and low-cost vaccine options.A major challenge is that the receptor-binding domain(RBD)-on
Long noncoding RNAs (lncRNAs) are emerging as a new class of important regulators of signal transduction in tissue homeostasis and cancer development.Liquid-liquid phase separation (LLPS) occurs in a wide range of biological processes,while its role in si
Dear Editor,rnThe spread of the SARS-CoV-2 variants,especially the global variants of concern (VOCs),could seriously dampen our efforts to tackle the COVlD-19 pandemic.The SARS-CoV-2 spike protein recognizes the host angiotensin-converting enzyme 2 (ACE2)
Dear Editor,rnTAR DNA-binding protein 43 kDa(TDP-43)undergoes liquid-liquid phase separation(LLPS)and forms reversible,cytoprotective nuclear bodies(NBs)under stress.1 Abnormal liquid-to-solid phase transition condenses TDP-43 into irreversible pathologic