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目的研究热休克蛋白gp96-肽复合物修饰树突状细胞(DC)后对人肝癌细胞的体外特异抗肿瘤效应。方法从人原发性肝癌组织中提取gp96-肽复合物,用其修饰DC细胞后与T淋巴细胞共同培养,以MTT法检测该复合物介导的细胞毒性T细胞(CTL)对不同来源人肝癌细胞的抗肿瘤效应,并与粗提抗原修饰DC细胞组相比较。结果 gp96-肽复合物诱导的CTL对原代肝癌细胞杀伤效率为74.3%,明显高于粗提抗原组(42.5%)和未加抗原组(14.4%)(P<0.01),粗提抗原组对肝癌细胞杀伤效率高于未加抗原组(P<0.01)。且该复合物的抗肿瘤效应具有一定的组织特异性。结论 gp96-肽复合物修饰的DC细胞,在体外能刺激产生特异的抗肿瘤细胞毒性T细胞,因而热休克蛋白gp96在肿瘤免疫治疗中具有重要的实用价值。
Objective To study the in vitro anti-tumor effect of heat shock protein gp96-peptide complex modified dendritic cells (DCs) on human hepatocellular carcinoma cells in vitro. METHODS: The gp96-peptide complex was extracted from human primary hepatocellular carcinoma (HCC) tissues and modified DCs were co-cultured with T lymphocytes. The cytotoxic T lymphocytes (CTLs) mediated by the complex were detected by MTT assay. The antitumor effect of hepatocarcinoma cells was compared with that of crude extract modified DC cells. Results The cytotoxicity of CTL induced by gp96-peptide complex on primary hepatocarcinoma cells was 74.3%, significantly higher than that of crude antigen group (42.5%) and non-antigen group (14.4%) (P <0.01) The killing rate of hepatoma cells was higher than that without antigen (P <0.01). The anti-tumor effect of the complex has certain tissue specificity. Conclusion gp96-peptide complex-modified DCs can stimulate specific anti-tumor cytotoxic T cells in vitro, so heat shock protein gp96 has important practical value in tumor immunotherapy.