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目的:探讨谷胱甘肽转硫酶(GSTs)基因多态性与浙江汉族溃疡性结肠炎(UC)易感性的关系.方法:选取浙江省温州医学院附属第二医院和温州市其他大型综合性医院UC患者252例,对照组共578例,系同期温州医学院附属第二医院体检中心的健康体检者.采用聚合酶链反应技术检测GST(M1、T1、P1)基因多态性在UC患者和正常对照者之间的分布差异.结果:UC患者中GSTM1(-)、GSTT1(-)和GSTP1(VaL/VaL)基因型频率明显高于正常对照组(69.45%vs46.30%,P=0.0003;61.51%vs51.39%,P=0.007;48.81%vs34.61%,P<0.0001).进一步根据UC临床症状分层分析,在远端结肠炎患者中GSTT1(-)、GSTP1(VaL/VaL)基因型的分布频率高于广泛性结肠炎患者(P=0.0001,P=0.001);而GSTM1(-)基因型与UC的病变部位无相关性(P=0.108).并且GST(M1、T1、P1)突变基因型与UC患者病情严重程度无关(均P>0.05).结论:GST(M1、T1、P1)基因突变与浙江汉族UC明显相关.
Objective: To investigate the relationship between glutathione S-transferase (GSTs) gene polymorphism and the susceptibility to ulcerative colitis (UC) in Zhejiang Han ethnicity.Methods: The Second Affiliated Hospital of Wenzhou Medical College of Zhejiang Province and other large-scale comprehensive 252 cases of UC patients in the sex hospital and 578 cases in the control group were all healthy subjects in the second affiliated hospital of Wenzhou Medical College during the same period.Using polymerase chain reaction to detect the polymorphisms of GST (M1, T1, P1) The frequencies of GSTM1 (-), GSTT1 (-) and GSTP1 (VaL / VaL) genotypes in patients with UC were significantly higher than those in normal controls (69.45% vs46.30%, P P <0.0001; P <0.0001; P <0.0001); P <0.0001; P <0.0001; P <0.0001; (P = 0.0001, P = 0.001). However, there was no correlation between GSTM1 (-) genotype and the lesion site of UC (P = 0.108), and the frequency of GST (M1 / VaL) genotype distribution was higher than that of patients with general colitis , T1, P1) had no correlation with the severity of UC (all P> 0.05) .Conclusion: Mutations of GST (M1, T1 and P1) are significantly associated with UC in Zhejiang province.