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目的:探讨TNF-α对小鼠B16黑色素瘤(MM)细胞凋亡的诱导及其对小鼠B16移植瘤生长的抑制作用。方法:用不同浓度的TNF-α直接作用于培养的小鼠B16黑色素瘤细胞,36h后,采用流式细胞仪(FCM)及原位末端标记法(TUNEL法)检测B16细胞的凋亡率。动物实验观察TNF-α小量瘤体内注射对小鼠B16移植瘤生长的抑制作用。结果:在1000、3000、5000及10000U/mLTNF-α作用下,B16细胞的凋亡指数均明显高于空白对照组(P<0.01),随着TNF-α浓度增加,B16凋亡细胞数呈增加趋势。动物实验结果显示,TNF-α治疗3周后,治疗组荷瘤小鼠MM移植瘤的体积和重量明显低于对照组(P<0.01)。结论:TNF-α能够诱导小鼠B16黑色素瘤细胞发生凋亡,且小剂量TNF-α瘤体内注射能显著抑制小鼠移植性MM的生长。
Objective: To investigate the induction of TNF-α on the apoptosis of mouse B16 melanoma (MM) cells and its inhibitory effect on the growth of mouse B16 xenografts. Methods: B16 melanoma cells were cultured with different concentrations of TNF-α. After 36 hours, the apoptosis rate of B16 cells was detected by flow cytometry (FCM) and TUNEL method. Animal experiments observed the small amount of tumor necrosis factor-αin vivo injection of B16 tumor growth inhibition. Results: Under the action of 1000, 3000, 5000 and 10000 U / mL TNF-α, the apoptosis index of B16 cells was significantly higher than that of the blank control group (P <0.01). With the increase of TNF-α concentration, Increase the trend. The results of animal experiments showed that the volume and weight of MM xenografts of tumor-bearing mice in the treatment group were significantly lower than those in the control group (P <0.01) after 3 weeks of TNF-α treatment. Conclusion: TNF-α can induce the apoptosis of B16 melanoma cells in mice, and the injection of low dose TNF-α can significantly inhibit the growth of transplanted MM in mice.