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半滑舌鳎(Cynoglossus semilaevis)是我国名贵海水养殖鱼类之一,其独特的生殖生物学特性受到国内外学者关注。本文以山东半岛养殖半滑舌鳎为对象,侧重研究贮精囊形态结构,以及雌激素受体(ER)和雄激素受体(AR)在不同精巢发育期贮精囊中的免疫组织化学定位。结果表明:精巢前端为贮精囊,其长度与生精部几乎等长,当精巢接近性成熟和性成熟时贮精囊非常饱满;贮精囊由结缔组织隔膜分割成小室,在生殖期间小室内有大量的成熟精子;AR和ER阳性反应部位主要发生在贮精囊小室间的结缔组织细胞和间质细胞内,在精巢重复发育期和排精后,贮精囊被膜和隔膜呈现强AR和ER阳性反应;精子形成期和成熟期,贮精囊被膜和隔膜呈弱AR和ER阳性反应。AR和ER可能介导半滑舌鳎贮精囊黏液、酶类、蛋白质合成与分泌作用。
Cynoglossus semilaevis is one of the rare marine aquaculture fishes in China. Its unique reproductive biology has attracted the attention of domestic and foreign scholars. In this paper, we studied the morphology and structure of the seminal vesicles, and the immunohistochemical localization of estrogen receptor (ER) and androgen receptor (AR) in the seminal vesicles of different testis developmental stages. The results showed that: the front of the testis is a seminal vesicle, its length is almost as long as that of the spermatogenesis section, and the seminal vesicle is very full when the testis is close to sexual maturity and sexual maturity. The seminal vesicle is divided into small cells by the connective tissue septum. During the reproductive period, . The positive reaction sites of AR and ER mainly occurred in the connective tissue cells and interstitial cells between the seminal vesicle cells. After the testes were repeatedly developed and ejaculated, the seminal vesicles and septum showed strong AR and ER positive reaction. Sperm formation and maturation, seminal vesicle capsule and diaphragm were weak AR and ER positive reaction. AR and ER may mediate mucosal, enzymatic, and protein synthesis and secretion in seminal vesicles.