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为探索油茶育种新途径,我们于1976—1980年期间进行了油茶花药培养单倍体植株的研究,初获花药愈伤组织分化的绿芽点和根。现将试验结果报道如下。一、材料与方法1.试验材料:供试材料为本所油茶林中定选优株和优良类型。接种花药的花粉发育时期分别为四分体期、单核晚期和双核初期。2.花药接种方法:以油茶的优良单株或类型植株上采集未开放的花苞,进行花粉发育时期的鉴定,然后置70%酒精液中表面消毒半分钟,捞出置0.1%升汞水中灭菌10分钟,用无菌水漂洗3—4次,放入接种箱或超净工作台上备用。3.培养基的配制:先后用N_6、Ms、B_5、MB、Miller和White等作基本培养基,按照不同组合配制诱导基43种,配制分化基39种。1979年底和1980年初,在综合前段试验的基础
In order to explore a new way for the breeding of Camellia oleifera, we studied the haploid plants of Camellia oleifera from 1976 to 1980 and obtained the green shoots and roots differentiated from anther callus. The test results are reported below. First, the materials and methods 1. Test materials: The test materials for the firm selected Camellia oleifera forest and excellent type. Anther pollen development period were tetrad, mononuclear and early binuclear. 2. Anther inoculation method: Take the non-open flower buds on the excellent single plant or type plant of Camellia oleifera for the identification of the pollen development stage, then sterilize the surface of 70% alcohol liquid for half a minute, remove and dispose of 0.1% Bacteria 10 minutes, rinsed with sterile water 3-4 times, into the inoculation box or clean bench spare. Preparation of culture medium: N6, Ms, B5, MB, Miller and White were used as the basic medium successively, and 43 kinds of induction bases were formulated according to different combinations, and 39 kinds of differentiation bases were prepared. The end of 1979 and the beginning of 1980, based on a comprehensive test before