本文应用反转录-聚合酶链反应成功的扩增了人谷胱甘肽过氧化物酶的互补脱氧核糖核酸(cDNA)。该法首先从人肝癌细胞中提取信使核糖核酸,在反转录酶的作用下生成cDNA,以此CDNA作为PCR的模板,利用人工合成的两个特异引物,进行35次循环的扩增。电泳结果表明,扩增片段的大小约为600 bp,与文献结果一致,为该酶基因的表达及生产奠定了基础。 In this paper, we successfully amplified the human glutathione peroxidase complementary deoxyribonucleic acid (cDNA) by reverse transcription - polymerase chain reaction. The method first extracts messenger RNA from human hepatoma cells and generates cDNA under the action of reverse transcriptase. Using CDNA as a template for PCR, amplification is performed for 35 cycles by using two synthetic primers. The results of electrophoresis showed that the size of the amplified fragment was about 600 bp, which was consistent with the results of the literature and laid the foundation for the expression and production of the enzyme gene.