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目的:探讨喉鳞状细胞癌(LSCC)组织中内皮依赖性血管和血管生成拟态(VM)的存在及其与临床病理学特征之间的关系。方法:采用组织芯片技术,进行苏木精-伊红染色、CD34免疫组织化学染色以及CD34/过碘酸雪夫双重染色技术检测138例喉癌组织中内皮依赖性血管和VM的表达。分析喉癌组织中内皮依赖性血管、VM与临床病理学特征之间的关系。结果:LSCC中存在VM的比例为26.23%(32/122),肿瘤微血管密度(MVD)平均值为12.61个/高倍视野。不同年龄、性别、临床分型、临床分期、病理分级、T分期及N分期,VM的阳性表达以及肿瘤MVD的表达差异均无统计学意义(P>0.05),但≥60岁年龄组VM和MVD均略高于<60岁组,低分化组VM和MVD略高于高分化+中分化组,N1~3组VM和MVD高于N0组。喉癌组织中VM的表达与MVD存在正相关关系(r=0.1927,P<0.05)。表明年龄越大、组织分化越差、分期越高,组织中VM及内皮依赖性血管的表达呈现升高的趋势。结论:VM是喉癌中存在的一种血液供应模式,VM的存在可能与喉癌的复发和转移有关。
Objective: To investigate the relationship between endothelium-dependent vasculogenic and angiogenic mimicry (VM) and clinicopathological features in laryngeal squamous cell carcinoma (LSCC). Methods: Tissue microarray was used to detect the expression of endothelium-dependent vasculature and VM in 138 laryngeal carcinoma tissues by hematoxylin-eosin staining, CD34 immunohistochemistry and CD34 / periodic acid Schiff’s dual staining. To analyze the relationship between endothelium-dependent vascular and VM and clinicopathological features in laryngeal cancer. Results: The proportion of VM in LSCC was 26.23% (32/122). The average microvessel density (MVD) in tumor was 12.61 / high power field. There was no significant difference in the expression of VM and the expression of MVD in different age, gender, clinical classification, clinical stage, histological grade, T stage and N stage (P> 0.05) MVD were slightly higher than <60 years old group. VM and MVD in poorly differentiated group were slightly higher than those in well differentiated and moderately differentiated group. VM and MVD in N1 ~ 3 group were higher than those in N0 group. There was a positive correlation between the expression of VM and MVD in laryngeal carcinoma (r = 0.1927, P <0.05). The results showed that the older, the worse the tissue differentiation, the higher the staging, the expression of VM and endothelium-dependent blood vessels in the tissues showed an increasing trend. Conclusion: VM is a blood supply model in laryngeal carcinoma. The presence of VM may be related to the recurrence and metastasis of laryngeal carcinoma.