论文部分内容阅读
采用构建16S rDNA克隆文库方法研究了渤海湾潮间带沉积物受多环芳烃(PAHs)荧蒽污染后细菌种群结构的变化。分别随机测定了对照组和实验组100个克隆子序列和50个克隆子序列(1 500 bp),Blast比对结果发现,对照组微生物群落多样性较高,可分为9大类群,主要优势种为不可培养细菌类群(uncultured bacterium,52%)、拟杆菌(Bacterioidates,21%)和变形菌群(Proteobacteria,21%);受荧蒽污染实验组中群落分为7大类群,主要优势种为不可培养细菌类群(70%)和变形菌群(18%)。另外,还发现降解石油和多环芳烃类污染物的相关微生物菌株明显增多。结果表明荧蒽污染明显改变了潮间带沉积物中的细菌种群结构,且导致相关降解菌株种类和数量增多。
The 16S rDNA clone library was used to study the changes of bacterial population structure in the intertidal sediments of the Bohai Gulf after being polluted by polycyclic aromatic hydrocarbons (PAHs). 100 clones and 50 clones (1 500 bp) were randomly selected from the control group and the experimental group respectively. Blast comparison showed that the microbial community in the control group had high diversity and could be divided into 9 groups. The main advantages The uncultured bacterium (52%), Bacterioidates (21%) and Proteobacteria (21%) were classified into 7 groups according to fluoranthene contamination. The dominant species The uncultured bacterial population (70%) and the deformed flora (18%). In addition, it has also been found that there is a significant increase in related microbial strains that degrade petroleum and PAHs. The results showed that fluoranthene pollution significantly changed the bacterial population structure in the intertidal sediments and resulted in an increase in the number and types of related degrading strains.