以我国特有的疣粒野生稻成熟胚和幼穗作外植体诱导愈伤组织 ,挑选致密颗粒状愈伤组织建立胚性细胞悬浮系并分离原生质体 ,将原生质体固体包埋后添加液体培养基进行培养。在培养过程中调节培养体系渗透压诱导产生胚状体 ,成功得到疣粒野生稻的原生质体再生植株 ,对提高原生质体再生频率的途径进行了探讨。 Induction of callus by explants of mature embryos and young ears of Warty Wild Rice (Oryza rufipogon Griff.) In China, selection of dense granule callus, establishment of embryogenic cell suspension and protoplast isolation, solid protoplast entrapment followed by liquid culture Base for training. In the process of culture, the osmotic pressure was used to regulate the culture system to induce the embryoid body, and the regeneration of protoplast from Warty wild rice was successfully obtained. The way to improve the regeneration frequency of protoplasts was also discussed.