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目的 研究 p38丝裂原激活蛋白激酶 (MAPK)选择性抑制剂SB2 0 35 80对乳鼠小脑颗粒神经元凋亡的保护作用。方法 SD乳鼠小脑颗粒神经元培养 ,琼脂糖凝胶电泳 ,SAPK/JNK分析试剂盒作激酶分析。结果 PI 3 K的特异性抑制剂LY2 940 0 2诱导小脑颗粒神经元凋亡 ,但SB2 0 35 80通过抑制细胞凋亡而促进小脑颗粒神经元的存活 ,且有浓度依赖性。LY2 940 0 2诱导凋亡的颗粒神经元中c Jun的表达量和磷酸化水平均升高 ,JNK被激活。但是 ,当小脑颗粒神经元生长在含SB2 0 35 80的高钾培养基中 ,c Jun的表达量、磷酸化水平和JNK的活性都明显的降低。结论 SB2 0 35 80通过抑制JNK的活性 ,降低c Jun的表达和磷酸化水平 ,对小脑颗粒神经元产生保护作用
Objective To study the protective effect of SB203580, a selective inhibitor of p38 mitogen-activated protein kinase (MAPK), on the apoptosis of cerebellar granule neurons in neonatal rats. Methods SD rat cerebellar granule neurons were cultured, agarose gel electrophoresis and SAPK / JNK assay kit were used for kinase analysis. Results LY2 940 0 2, a specific inhibitor of PI 3 K, induces apoptosis of cerebellar granule neurons. However, SB2 0 35 80 promotes the survival of cerebellar granule neurons by inhibiting apoptosis, in a concentration-dependent manner. The expression and phosphorylation of c Jun in granulosa neurons induced by LY2 940 02 increased, and JNK was activated. However, when cerebellar granule neurons grew in high potassium medium containing SB203580, the expression level of c Jun, phosphorylation and JNK activity were significantly reduced. Conclusion SB2 0 35 80 can protect cerebellar granule neurons by inhibiting the activity of JNK, decreasing the expression of c Jun and phosphorylation