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目的:利用胰腺发育相关基因PDX1-诱导小鼠胎肝基质细胞向胰腺细胞分化,探讨糖尿病治疗新方法。方法:构建含有胰腺发育相关基因胰腺-十二指肠同源框1(pancreatic duodenal hom eobox1,PDX1-)的真核细胞表达载体pmPDX1-,瞬时转染小鼠胎肝基质细胞,通过RT-PCR的方法,检测转染pmPDX1-质粒的小鼠永生化胎肝基质细胞的胰岛素、胰高血糖素、胰多肽和肝细胞生长因子受体c-m et的表达。结果:在mRNA水平上能够检测到胰岛β细胞所分泌的胰岛素、胰岛α细胞所分泌的胰高血糖素和胰腺PP细胞所分泌的胰多肽,表明转染了pmPDX1-质粒的小鼠胎肝基质细胞在向胰腺细胞分化的过程中,具备了转录表达胰腺的内外分泌因子的能力。对肝细胞生长因子受体c-m et的检测,提示分化的细胞没有完全失去肝细胞的特性。结论:通过导入PDX1-基因可以诱导小鼠胎肝基质细胞向胰腺细胞分化。
OBJECTIVE: To use PDX1-induced pancreatic development-related gene to induce mouse fetal liver stromal cells to differentiate into pancreatic cells, and to explore a new method for the treatment of diabetes. Methods: The eukaryotic expression vector pmPDX1 - containing pancreatic duodenal homoeobox1 (PDX1 -) was constructed and transiently transfected into mouse fetal liver stromal cells. The expression of insulin, glucagon, pancreatic polypeptide and hepatocyte growth factor receptor cm et was measured in mouse immortalized fetal liver cells transfected with pmPDX1-plasmid. RESULTS: Insulin secreted by pancreatic β cells, glucagon secreted by pancreatic α cells and pancreatic polypeptide secreted by pancreatic PP cells were detected at the mRNA level, indicating that the pmPDX1-plasmid transfected mouse fetal liver matrix Cells in the process of differentiation into the pancreas, with transcriptional expression of pancreatic secretion of internal and external factors. The detection of hepatocyte growth factor receptor c-m et, suggesting that the differentiated cells did not completely lose the characteristics of liver cells. Conclusion: PDLC1 gene can induce the differentiation of mouse fetal liver stromal cells into pancreatic cells.