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利用表达性差异显示分析 (RDA)技术 ,研究大鼠 2 /3肝部分切除后 1h肝组织中基因的选择性表达 ,建立了大鼠 2 /3肝部分切除术后 1h再生肝组织选择性表达基因EST库。该库约含 3× 10 4 个独立克隆 ,其中 95 %以上的克隆含有插入片段 ,长度约 2 0 0～ 70 0bp不等 ,对随机挑出的 5 2个克隆的序列分析表明其中大多数基因与肝再生调控相关 (38/5 2 )。 10株未报道序列经RNA杂交证实 ,其中 6株与肝再生相关。
The expression of genes was selectively detected in hepatic tissues 1 / 2h after partial hepatectomy in rats by expression differential display analysis (RDA), and the selective expression of hepatic tissue was established 1 / 2h after partial hepatectomy in rats Gene EST library. The library contains about 3 × 10 4 independent clones, of which 95% of the clones contain the inserted fragments ranging in length from 200 to 70 0 bp. The sequence analysis of randomly selected 52 clones showed that most of the genes Regulated by liver regeneration (38/5 2). Ten unreported sequences were confirmed by RNA hybridization, of which six were associated with liver regeneration.