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为建立仓储阶段转Bt水稻安全性评价中靶标害虫抗性汰选研究体系,配制了含不同比例(70%,50%,30%,10%)转Bt基因(Cry1Ab/Cry1Abc)明辉63水稻谷粉(简称Bt谷粉)的人工饲料饲喂印度谷螟Plodia interpunctella(Hübner),测定其对1～3龄幼虫在72h内的急性毒力,及对印度谷螟种群生长发育的影响,并采用ELISA法检测转基因稻谷和末龄幼虫体内Bt蛋白含量。结果发现:4种比例人工饲料对幼虫的毒力作用均发生在取食48h后,72h后剂量效应明显。含Bt水稻较高比例的饲料对印度谷螟发育的负面效应明显:幼虫死亡率高,发育历期延长。Bt蛋白在幼虫体内含量与对应饲料中的含量基本成正比。综合考虑,将Bt杀虫蛋白含量2.35μg/g作为转Bt基因稻谷对印度谷螟的亚致死剂量最为合适。
In order to establish a research system of target pest resistance selection in the safety evaluation of transgenic Bt rice in storage stage, the transgenic Bt gene (Cry1Ab / Cry1Abc) with different proportions (70%, 50%, 30%, 10% The artificial feed of rice flour (Bt) was fed on the Plodia interpunctella (Hübner). The acute toxicity to the first to third instar larvae in 72 h and the effect on the population growth and development of the Indian moth The contents of Bt protein in transgenic rice and the last instar larvae were detected by ELISA. The results showed that the toxic effects of four kinds of artificial feed on larvae all occurred after 48 hours of feeding, and the dose effect was obvious after 72 hours. The negative effects of a higher proportion of Bt-containing rice diets on the development of the Indian moth borer were significant: larval mortality was high and developmental duration was prolonged. The content of Bt protein in larvae is basically proportional to the content of the corresponding feed. Taken together, the Bt insecticidal protein content of 2.35μg / g as Bt transgenic rice on the Indian Stem boreal sublethal dose most appropriate.