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紫外辐射能显著地降低人参培养细胞单细胞克隆的植板率。当紫外辐射悬浮细胞30s后,细胞克隆的植林率是对照组的21.43%。细胞克隆平板培养60d,挑取克隆连续转移培养3次,共获得克隆系122株。对所有克隆系进行变异分析并经10代连续继代培养,从中筛选到一株稳定的高产寡糖素克隆系PGUA-08,而且它的过氧化物酶同工酶谱特征也保持稳定。克隆系PGUA-08的生长速率为0.537gDWL-1d-1,是亲本的1.46倍,寡糖素含量为17.16%DW,是亲本的1.81倍,寡糖素产率为2.764g/L,是亲本的2.62倍。
UV radiation can significantly reduce the ginseng cultured cells unicellular clonal plating rate. When the cells were suspended by ultraviolet radiation for 30s, the rate of cell colonization was 21.43% of the control group. The cell clone plate culture 60d, picked the clone continuous transfer culture three times, a total of 122 clone lines were obtained. All of the clones were subjected to mutation analysis and subcultured successively for 10 generations, and a stable high-yielding oligosaccharides clone PGUA-08 was screened out, and its peroxidase isozyme profile was also stable. The growth rate of clone PGUA-08 was 0.537gDWL-1d-1, which was 1.46 times of that of the parent, the content of oligosaccharides was 17.16% DW, which was 1.81 times of the parent, the yield of oligosaccharides was 2.764g / L, 2.62 times that of the parent.