论文部分内容阅读
大鼠姊妹染色单体交换(SCE)分析是检测遗传毒性的重要指标之一,但以往的方法是从心脏或眼球静脉丛取血,不仅用血量大,而且还有易污染、不安全等缺点.为此,我们参照大鼠尾血染色体技术,改用昆血进行SCE分析,并应用于1,2-二氯乙烷染毒大鼠SCE测定,获得满意结果。该法取血方便,用血量少,仅用2～3滴,不用清洗血去除抑制因子,减少了污染机会,只由培养基稀释直接培养便能得到较多分裂相。现介绍如下:
Sister chromatid exchange (SCE) analysis in rats is one of the important indicators of genotoxicity detection, but the previous method is to take blood from the heart or the ophthalmic venous plexus, not only with the large amount of blood, but also easily contaminated, unsafe For this reason, we refer to the rat tail blood chromosome technology, instead of using Kunming blood for SCE analysis, and applied to 1,2-dichloroethane exposure rats SCE determination, to obtain satisfactory results. The method of convenient blood, with less blood, only 2 to 3 drops, without cleaning the blood to remove the inhibitory factor, reducing the chance of contamination, only by medium dilution direct cultivation can get more split phase. Now introduced as follows: