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乳腺癌是西方女性最常见的恶性病变,改善治疗效果的关键在于了解肿瘤的生物学特性,作者研究了59例原发性乳腺癌病人的DNA倍体类型和临床、生化以及病理特性的关系。作者于1978～1979年分析59例乳腺癌根治术病人的标本。临床分期用UICC标准,肿块大小根据TNM分期标准,全部病人做了改良的根治切除术,术后均定期做临床、放射学和生化检查,随访至少4年或至病人死亡。将手术标本用机械法离散后再用70μ的尼龙网过滤,最后用70%酒精固定后,4℃保存。在流式细胞仪(FCM)检测前先用生理盐水冲洗,再用0.1%RNA酶温育(37℃)1小时,以及0.4%的胃蛋白酶
Breast cancer is the most common malignancy in women in the West. The key to improving treatment is to understand the biological characteristics of the tumor. The authors studied the relationship between DNA ploidy and clinical, biochemical, and pathological features in 59 patients with primary breast cancer. The authors analyzed specimens of 59 patients undergoing radical mastectomy from 1978 to 1979. Clinical staging was based on the UICC criteria. The size of the mass was based on the TNM staging criteria. All patients underwent an improved radical resection. All patients underwent clinical, radiological, and biochemical tests on a regular basis and were followed up for at least 4 years or until the death of the patient. The surgical specimens were mechanically dissociated and then filtered through a 70μ nylon mesh, and finally fixed with 70% alcohol and stored at 4°C. Rinse with saline before testing by flow cytometry (FCM), incubate (37°C) for 1 hour with 0.1% RNase, and 0.4% pepsin