血清肌酸测定通常采用间接测定法,即无蛋白血滤液经加酸加热处理,使肌酸脱水产生肌酐,再由测定加热前后肌酐之差值求得肌酸含量。由于需要两次测定,干扰因素较多,往往影响结果的准确性。我们参考有关资料,采用肌酸与α-萘酚和丁二酮在碱性条件下显色原理,应用于血清肌酸直接测定,同时做了105例成年人正常参考值,现介绍如下。 Serum creatinine determination is usually used indirect method, that is, protein-free blood filtrate by adding acid heat treatment, the creatine dehydration produce creatinine, and then measured by the creatinine difference between before and after creatine content. Due to the need for two determinations, more interference factors often affect the accuracy of the results. We refer to the relevant information, the use of creatine and α-naphthol and diacetyl in alkaline conditions under the principle of color, used in direct determination of serum creatinine, while doing the normal reference value of 105 adults, are described below.