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目的研究乙肝疫苗接种后无弱应答的发生与IL-2或IL-6水平之间的关系,从分子生物学角度探讨无弱应答者发生的分子生物学机制。方法从健康人群中随机选取30个乙肝疫苗接种后无弱应答者和正常应答者作为本次研究的对象。采用CTLL-2和7TD1方法监测IL-2和IL-6活性水平。比较两组IL-2和IL-6活性水平,并建立起IL-2和IL-6活性水平与抗-HBs滴度之间的回归方程,以通过IL-2和IL-6活性水平来预测乙肝疫苗接种后无弱应答的发生。结果乙肝疫苗接种后无弱应答的IL-2活性水平的平均值明显低于正常应答者(P<0.01),无弱应答的IL-2活性水平和正常应答者的平均值分别为74.5±64.1u/ml和298.0±101.1u/ml。并且相关回归分析发现,在乙肝疫苗接种后正常应答者组中抗-HBs滴度与IL-2活性水平之间呈明显正相关,相关系数为r=0.68。正常应答者组的IL-6活性水平(58.98±16.4)明显高于无弱应答者组(30.10±12.4)(P<0.05),而且在乙肝疫苗接种后正常应答者组中抗-HBs滴度也与IL-6活性水平之间呈明显正相关,相关系数为0.79(P<0.05)。结论乙肝疫苗接种后无弱应答者的发生可能与被接种者体内IL-2、IL-6活性水平有关。提示我们,为了降低乙肝疫苗接种后无弱应答者的发生率,可以在注射乙肝疫苗的同时注射少量IL-2或IL-6。
Objective To study the relationship between the occurrence of non-weak response and the level of IL-2 or IL-6 after hepatitis B vaccination and to explore the molecular biological mechanism of non-responders from the perspective of molecular biology. Methods Thirty randomly selected subjects from the healthy population who were not weakened responders and normal responders after vaccination were used as the study subjects. IL-2 and IL-6 activity levels were monitored using the CTLL-2 and 7TD1 methods. IL-2 and IL-6 activity levels were compared between the two groups and a regression equation between IL-2 and IL-6 activity levels and anti-HBs titer was established to predict the level of IL-2 and IL-6 activity No weak response after hepatitis B vaccination. Results The average level of IL-2 activity without weak response after hepatitis B vaccination was significantly lower than that of normal responders (P <0.01). The average level of IL-2 activity of non-responders and normal responders were 74.5 ± 64.1 u / ml and 298.0 ± 101.1 u / ml. Correlation analysis showed that there was a positive correlation between anti-HBs titer and IL-2 activity in the normal responders group after hepatitis B vaccination, with a correlation coefficient of r = 0.68. The level of IL-6 in the normal responders group (58.98 ± 16.4) was significantly higher than that in the non-responders group (30.10 ± 12.4) (P <0.05), and the anti-HBs titer in the normal responders group There was also a significant positive correlation with IL-6 activity, with a correlation coefficient of 0.79 (P <0.05). Conclusion The occurrence of non-responders after hepatitis B vaccination may be related to the levels of IL-2 and IL-6 in vaccinated patients. It is suggested that in order to reduce the incidence of non-responders after hepatitis B vaccination, a small amount of IL-2 or IL-6 may be injected with hepatitis B vaccine.