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目的对舟山眼镜蛇毒α-神经毒素的致突变性进行研究,为临床应用的安全性提供依据。方法应用中国仓鼠肺成纤维细胞CHL染色体畸变试验和Ames试验检测舟山眼镜蛇毒α-神经毒素是否有致突变性。结果 Ames试验:对于突变株TA97、TA100和TA102,α-神经毒素浓度达到或者超过2.56μg·m L-1,Ames试验阳性(P<0.05);对于突变株TA98,α-神经毒素浓度达到或者超过5.12μg·m L-1,Ames试验阳性(P<0.05)。但回复突变和畸变率与神经毒素的浓度不呈线性关系,也与是否有活化剂S9无关联。染色体畸变试验:α-神经毒素浓度达到或者超过0.64μg·m L-1,CHL细胞染色体畸变为阳性(P<0.05),但畸变率与神经毒素的浓度不呈线性关系,也与是否有活化剂S9无关联。结论舟山眼镜蛇毒α-神经毒素临床剂量为1μg·kg·d-1时在致突变性上是安全的。
Objective To study the mutagenicity of α-neurotoxin in Naja naja at Zhoushan, and provide the basis for the safety of clinical application. Methods CHL chromosomal aberration test of Chinese hamster lung fibroblasts and Ames test were used to detect the mutagenicity of α-nerve toxin of Zhoushan Cobra venom. Results Ames test: The Ames test was positive (P <0.05) for α-neurotoxins at or above 2.56 μg · m L-1 for mutant strains TA97, TA100 and TA102; for mutant TA98, α-neurotoxin concentrations reached or More than 5.12μg · m L-1, Ames test was positive (P <0.05). However, there was no linear relationship between the rate of mutation and the rate of mutation and the concentration of neurotoxin, and no correlation with the presence or absence of activator S9. Chromosome aberration test: Chromosome aberration was positive in CHL cells (P <0.05) at α-neurotoxin concentrations of 0.64 μg · mL -1 or more, but there was no linear relationship between the aberration rate and the concentration of neurotoxin and with or without activation S9 No correlation. Conclusion The neurotoxicity of α-neurotoxin of Zhoushan Cobra venom at 1 μg · kg · d-1 is safe in mutagenicity.