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本文建立了简便、灵敏的定量分析血浆中盐酸纳洛酮和盐酸纳曲酮的高效液相色谱－电化学检测法。血浆样品用苯－乙醇（９∶１）混合溶剂提取，在Ｃ１８柱上以０．１ｍｏｌ／Ｌ磷酸二氢钾－甲醇（８４∶１６）为流动相进行分离，用电化学检测器在７６０ｍＶ的工作电压下进行检测，色谱峰分离完全，血浆本底无干扰。将盐酸纳曲酮和盐酸纳洛酮互为内标，测得两药在２．５～１６０ｎｇ／ｍｌ的范围内具有良好的线性关系，血浆中盐酸纳洛酮和盐酸纳曲酮的回收率分别为７６．８％～８９．６％和７８．６％～８４．６％，日内ＲＳＤ＜４．８％，日间ＲＳＤ＜９．１％，最低检出浓度为１ｎｇ／ｍｌ血浆。应用本法对犬ｉｖ盐酸纳洛酮和盐酸纳曲酮的药代动力学进行了研究，报告了相应的动力学参数。
This article established a simple and sensitive quantitative analysis of plasma naloxone hydrochloride and naltrexone hydrochloride by high performance liquid chromatography - electrochemical detection. The plasma samples were extracted with a mixed solvent of benzene and ethanol (9: 1) and separated on a C18 column with 0.1 mol / L potassium dihydrogen phosphate-methanol (84:16) as the mobile phase. The electrochemical detector was operated at 760 mV Under the working voltage test, the chromatographic peak is completely separated and the plasma background has no interference. Naltrexone hydrochloride and naloxone hydrochloride internal standard for each other, measured in the range of 2.5 ~ 160ng / ml of the two drugs has a good linear relationship between the plasma naloxone hydrochloride and naltrexone hydrochloride recovery Respectively, ranging from 76.8% to 89.6% and 78.6% to 84.6% respectively. The intraday RSD was less than 4.8%, the intraday RSD was less than 9.1%, and the lowest detected concentration was 1ng / ml plasma. The pharmacokinetics of naloxone hydrochloride and naltrexone hydrochloride in dogs iv were studied by this method and the corresponding kinetic parameters were reported.