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应用大鼠皮肤原代细胞培养并结合ＭＴＴ法检测氧化型染发剂对皮肤细胞的毒性和增殖能力的影响，结果表明，对苯二胺经双氧水氧化后形成的化合物以及Ｇ牌染发剂均对大鼠角朊细胞的生长有抑制作用，随着染毒浓度的增高，ＯＤ值逐渐降低，即细胞线粒体代谢ＭＴＴ成ＭＴＴ甲月赞的能力降低，且具有剂量反应关系（ｒ对苯二胺和双氧水混合物＝－０６８７，ｒＧ牌染发剂＝－０９１３，ｎ＝３０，Ｐ＜００１）。除最低剂量组以外，其它４个浓度组的ＯＤ值均数与对照组相比均显著降低（ｔ检验，Ｐ＜００１）。对苯二胺和双氧水混合物及Ｇ牌染发剂的ＩＣ５０分别为０００３３％和００３９２ｍｇ／ｍｌ（９５％可信区间分别为０００２１％～０００５２％及００２０９～００７３５ｍｇ／ｍｌ）。结果显示对苯二胺与双氧水混合物以及Ｇ牌染发剂均具有较强的细胞毒性作用，能够抑制细胞增殖。氧化型染发剂的细胞毒性可能与其引起皮肤损伤的机制有关。
The effects of oxidative hair dye on the skin cells toxicity and proliferation were tested by primary skin cell culture and MTT assay. The results showed that the compounds of the phenylenediamine oxidized by hydrogen peroxide and the G brand hair dye were both With the increase of exposure concentration, the OD value decreased gradually, that is, the mitochondrial metabolism of MTT reduced the ability of MTT-A-C and had a dose-response relationship (r-phenylenediamine and Hydrogen peroxide mixture = -0687, rG brand hair dye = -0.913, n = 30, P <001). Except for the lowest dose group, OD values of the other four concentration groups were significantly lower than those of the control group (t test, P <001). The IC50 of p-phenylenediamine and hydrogen peroxide mixture and G brand hair dye were 00033% and 00392 mg / ml, respectively (95% confidence interval were 00021% ~ 00052% and 00209 ~ 0 0735 mg / ml). The results show that p-phenylenediamine and hydrogen peroxide mixture and G brand hair dye have a strong cytotoxicity, can inhibit cell proliferation. The oxidative hair dye cytotoxicity may be related to its mechanism of causing skin damage.