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目的 探讨转化生长因子 β1、β3 (TGF β1、β3 )及其受体 βRⅠ、βRⅡmRNA与子宫肌瘤发生发展的关系。方法 2 0 0 0年 12月至 2 0 0 1年 11月采用免疫组化及原位杂交染色方法 ,对 30例子宫肌瘤及正常子宫肌组织标本检测TGF β1、β3 及其受体 βRⅠ、βRⅡmRNA的表达。 结果 (1)子宫肌瘤组织中TGF β1、β3 蛋白表达强于邻近正常肌层 (P <0 0 1) ,且黄体期较卵泡期增高 (P <0 0 1)。TGF βRⅠ水平较邻近正常肌层亦增高 (P <0 0 1) ,TGF βRⅡ则下降 (P <0 0 5 )。 (2 )原位杂交TGF β1、β3 mRNA杂交信号的检测结果与免疫组化检测结果一致。结论 TGF β1、β3 与子宫肌瘤的发生发展密切相关 ,TGF βRⅡ降表达或表达异常可能是TGF βs促进子宫肌瘤发生发展的始动环节
Objective To investigate the relationship between the expression of transforming growth factor β1, β3 (TGF β1, β3) and their receptors βR Ⅰ, β R Ⅱ mRNA and the occurrence and development of uterine fibroids. Methods From December 2000 to November 2001, immunohistochemistry and in situ hybridization staining were used to detect the expression of TGFβ1, β3 and its receptor βRⅠ in 30 cases of uterine leiomyoma and normal uterine muscle tissue specimens, βRII mRNA expression. Results (1) The expressions of TGFβ1 and β3 in uterine fibroids were significantly higher than those in adjacent normal myometrium (P <0.01), and the luteal phase was higher than that in follicular phase (P <0.01). TGFβRⅠlevels were also higher than those in adjacent normal myometrium (P <0.01), and TGFβRⅡdecreased (P <0.05). (2) The in situ hybridization TGFβ1, β3 mRNA hybridization signal test results and immunohistochemical test results. Conclusion TGF β1 and β3 are closely related to the occurrence and development of uterine leiomyoma, and the expression or abnormal expression of TGF β R Ⅱ may be the initiating part of TGF βs to promote the development of uterine fibroids