论文部分内容阅读
某些逆转录病毒(retrovirus)带有使细胞癌变的DNA序列,即为病毒的癌基因(v-oncogen或v-onc)。病毒癌基因与粒子病毒无关,不影响病毒复制,却与正常细胞的DNA序列同源,故可看作病毒的寄生物。目前已从诱生动物肿瘤的逆转录病毒中分离得十几种癌基因,以分离得的动物及所诱生肿瘤名称而命名。如大鼠Harvey肉瘤病毒的癌基因(V—Ha—ras)等。以病毒癌基因互补DNA(cDNA)为探针,使用DNA杂交及转染等技术,证实脊椎动物以至人类正常细胞的DNA中皆有
Some retroviruses carry DNA sequences that make the cells cancerous, ie the viral oncogenes (v-oncogen or v-onc). Viral oncogene has nothing to do with particle virus, does not affect virus replication, but is homologous with the DNA sequence of normal cells, so it can be considered as a virus parasite. More than a dozen oncogenes have been isolated from retroviruses that induce animal tumors, and are named after the isolated animals and the names of the induced tumors. Such as rat Harvey sarcoma virus oncogene (V-Ha-ras) and so on. Using viral oncogene-complementary DNA (cDNA) as a probe, DNA hybridization and transfection technologies have been used to confirm the presence of DNA in vertebrate and even normal human cells.