【摘 要】
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Nocardia, Rhodococcus and Streptoyces, all members of the actinomycetes family, are Gram-positive eubacteria with high G+C content and able to form mycelium. We
【机 构】
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College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China“,”Ins
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Nocardia, Rhodococcus and Streptoyces, all members of the actinomycetes family, are Gram-positive eubacteria with high G+C content and able to form mycelium. We report here a newly identified plasmid pXT107 of Nocardia sp. 107, one of the smallest circular plasmids found in Nocardia.The complete nucleotide sequence of pXT107 consisted of 4335 bp with 65% G+C content, and encoded one replication extragenic palindromic (Rep) and six hypothetical proteins. The Rep, double-strand origin and single-strand origin of pXT107 resembled those of typical rolling-circle-replication plasmids, such as pNI100 of Nocardia, pRE8424 of Rhodococcus and plJ101 of Streptomyces. The Escherichia coli-Nocardia shuttle plasmid pHAQ22, containing thc rep gene of pXT107, is able to propagate in Nocardia but not in Streptomyces.
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