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观察粉防己碱对高血压心肌肥厚大鼠心肌胶原含量和肌球蛋白ATP酶活性的影响 .采用二肾一夹肾血管性高血压造成大鼠左心室肥厚模型 ,自术后第 9周起按粉防己碱 50mg·kg- 1,依那普利 6mg·g- 1灌胃给药 ,每日 1次 ,连续 8周 .用分光光度法测定心肌羟脯氨酸含量及肌球蛋白ATP酶活性 .结果表明左心室肥厚组心肌羟脯氨酸为 (5.9± 0 .3)mg·g- 1干重 ,明显高于假手术对照组〔(3.6± 0 .4 )mg·g- 1干重〕 ,粉防己碱组和依那普利组心肌羟脯氨酸含量分别较左心室肥厚组低 2 8.2 %和 39.0 % .左心室肥厚组肌球蛋白ATP酶活性仅为 (0 .4 3± 0 .0 9)mmolPi·min- 1·g- 1蛋白质 ,较假手术对照组〔(0 .97±0 .0 6 )mmolPi·min- 1·g- 1蛋白质〕明显降低 ,而粉防己碱组和依那普利组则分别比左心室肥厚组高 6 0 .5%和 118.6 % .实验结果表明粉防己碱可部分逆转肾血管性高血压所致的大鼠左心室肥厚 ,降低心肌胶原含量 ,升高肌球蛋白ATP酶活性
To observe the effects of tetrandrine on myocardial collagen content and myosin ATPase activity in hypertensive rats with hypertrophic hearts.The model of left ventricular hypertrophy in rats with hypertensive renal hypertrophy was induced by Tetrandrine (50mg · kg-1) and enalapril 6mg · g-1 were given intragastrically once daily for 8 weeks.The contents of hydroxyproline and myosin ATPase in myocardium were measured by spectrophotometry The results showed that the myocardial hydroxyproline in the left ventricular hypertrophy group was (5.9 ± 0.3) mg · g -1 dry weight, which was significantly higher than that in the sham operation control group (3.6 ± 0.4 mg · g -1 dry weight ), The hydroxyproline content of myocardium in the tetrandrine group and the enalapril group were respectively lower than that of the left ventricular hypertrophy group by 8.22% and 39.0%, and the activity of myosin ATPase in the left ventricular hypertrophy group was only 0. 9) mmolPi · min-1 · g-1 protein was significantly lower than that of the sham operation control group [(0.97 ± 0.06) mmolPi · min -1 · g -1 protein) Group and enalapril group were higher than the left ventricular hypertrophy group 0.55% and 118.6% .Experimental results show that tetrandrine can partially reverse the renovascular hypertensive rats induced left ventricular hypertrophy and reduce myocardial Collagen content, increase myosin ATPase activity