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目的研究转化生长因子-β1(TGF-β1)、白细胞介素-1β(IL-1β)干预人牙周膜成纤维细胞后HGF的基因表达的水平变化,为HGF网络调控人牙周膜成纤维细胞功能的研究提供新资料。方法分别采用IL-1β梯度浓度、IL-1β最佳干预浓度和TGF-β1梯度浓度干预第5代人牙周膜成纤维细胞,并运用RT-PCR法检测人牙周膜成纤维细胞中HGF的基因表达。结果经IL-1β单独作用,人牙周膜成纤维细胞中HGF的基因表达水平上调,并优选出IL-1β的最佳干预浓度为10ng/ml(P<0.05);当一定浓度的TGF-β1和10ng/ml的IL-1β共同作用于人牙周膜成纤维细胞后,该细胞中HGF的基因表达水平比单独IL-1β作用组低(P<0.05)。结论 TGF-β1能够抑制IL-1β所诱导的人牙周膜成纤维细胞中HGF的基因表达。
Objective To study the changes of HGF gene expression in human periodontal ligament fibroblasts after the intervention of transforming growth factor-β1 (TGF-β1) and interleukin-1β (IL-1β) Cell function research to provide new information. Methods The 5th generation human periodontal ligament fibroblasts were treated with gradient IL-1β, IL-1β best concentration and TGF-β1 gradient respectively. RT-PCR was used to detect the expression of HGF in human periodontal ligament fibroblasts The gene expression. Results The gene expression of HGF in human periodontal ligament fibroblasts was up-regulated by IL-1β alone, and the optimal concentration of IL-1β was 10ng / ml (P <0.05). When a certain concentration of TGF- After co-administration of β1 and IL-1β at 10ng / ml to human periodontal ligament fibroblasts, the gene expression of HGF was lower than that of IL-1β alone (P <0.05). Conclusion TGF-β1 can inhibit the gene expression of HGF in human periodontal ligament fibroblasts induced by IL-1β.