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目的:探讨黄芪对急性肾缺血-再灌注大鼠肾功能、细胞因子、游离钙水平和细胞周期蛋白(Cyclin D1)的影响。方法:90只SD雄性大鼠随机分为肾缺血-再灌注组(模型组)、假手术组(对照组)、黄芪组(实验组)模型,分别于再灌注后3,6,12h各留取10只取血及肾组织标本,比较各组肾功能、细胞因子、游离钙水平和Cyclin D1水平。结果:1与对照组相比,模型组6h后血肌酐(Cr)、尿素氮(BUN)均明显增加(P<0.01);实验组6h后BUN明显增加,12h后Cr增加,与对照组相比差异具有统计学意义(P<0.05);6h后模型组与实验组Cr差异具有统计学意义(P<0.05);12h后模型组与实验组BUN差异具有统计学意义(P<0.05)。2再灌注3,6,12h后模型组和实验组IL-6均高于对照组(P<0.01),但实验组低于模型组(P<0.01);模型组6h后IL-10低于对照组和实验组(P<0.05),其他组别间差异无统计学意义(P>0.05);再灌注3,6,12h后模型组和实验组TNF-α均高于对照组(P<0.01),但实验组低于模型组(P<0.01)。3模型组和实验组的[Ca2+]i自3h后即明显高于对照组(P<0.01),但实验组3,6h水平低于模型组(P<0.01),12h模型组和实验组差异无统计学意义(P>0.05);Cyclin D1水平,3h后模型组高于对照组(P<0.05),实验组3h与模型组差异有统计学意义(P<0.05),6h后与模型组差异无统计学意义(P>0.05)。结论:黄芪能够减轻缺血-再灌注大鼠钙超载,调控细胞因子释放,具有减轻大鼠肾脏损伤、保护肾功能的作用。
Objective: To investigate the effects of Astragalus membranaceus on renal function, cytokines, free calcium and cyclin D1 in rats with acute renal ischemia-reperfusion. Methods: Ninety male Sprague-Dawley rats were randomly divided into 3 groups: model group, sham operation group (control group) and Astragalus mongholicus group (experimental group) Ten samples of blood and kidney tissue were collected and the renal function, cytokines, free calcium and Cyclin D1 were compared between groups. Compared with the control group, the levels of serum creatinine (Cr) and blood urea nitrogen (BUN) in model group increased significantly after 6h (P <0.01); the BUN in experimental group increased significantly after 6h, (P <0.05). After 6 hours, the difference between model group and experimental group was statistically significant (P <0.05). After 12 hours, the difference between model group and experimental group was statistically significant (P <0.05). The levels of IL-6 in model group and experimental group were significantly higher than those in control group (P <0.01) at 3, 6 and 12 h after reperfusion, but were lower in experimental group than in model group (P <0.01) (P <0.05). There was no significant difference among the other groups (P> 0.05). After reperfusion for 3, 6 and 12 hours, TNF-|Áof the model group and experimental group were higher than that of the control group (P < 0.01), but the experimental group was lower than the model group (P <0.01). The level of [Ca2 +] i in model group and experimental group was significantly higher than that in control group (P <0.01) after 3h, but the level of 3,6h in experimental group was lower than that in model group (P <0.01) (P <0.05). The level of Cyclin D1 in model group was higher than that in control group after 3h (P <0.05), and there was a significant difference between model group and experimental group at 3h (P <0.05) The difference was not statistically significant (P> 0.05). Conclusion: Astragalus can relieve calcium overload and regulate the release of cytokines in rats with ischemia-reperfusion injury, which can relieve kidney damage and protect renal function in rats.