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目的 了解GⅡ.4型诺如病毒不同变异株与受体人组织血型抗原(human histo-blood group antigens,HBGAs)的结合模式及HBGAs在GⅡ.4型诺如病毒进化过程中的角色.方法 利用RT-PCR方法扩增5株不同GⅡ.4型诺如病毒变异株的衣壳蛋白P区序列,在原核系统中表达并纯化P蛋白.通过一组HBGAs表型明确的唾液样本与P蛋白结合,进行唾液HBGAs结合模式分析.同时,将P蛋白与人工合成的HBGAs寡糖进行寡糖结合分析.结果 唾液HBGAs结合模式分析表明,Sakai变异株几乎不结合,其他GⅡ.4变异株的HBGAs结合模式相似,与分泌型唾液(A、B、AB和O型分泌型)结合,与O型非分泌型唾液不结合.95/96US变异株和2006b变异株结合能力较高,其次是Camberwell变异株,Hunter变异株较弱.寡糖结合分析与唾液结合分析结果一致,Sakai变异株不结合,其他毒株与均与分泌型寡糖(Ley和H1)结合,与非分泌型寡糖(Lea和Lex)不结合.95/96US变异株和2006b变异株与寡糖的结合能力较强,其次是Camberwell变异株.结论 绝大多数GⅡ.4型诺如病毒变异株与HBGAs结合模式相同,但结合能力不同,结合能力强的变异株流行范围较广.“,”Objective To investigate the binding profile of the epochal GⅡ.4 norovirus (NoV) variants to the human histo-blood group antigens (HBGAs) and the role of HBGAs in the evolution of the GⅡ.4 NoVs.Methods The sequences of P domain in capsid region of 5 GⅡ.4 NoV variants were amplified by RT-PCR,and P proteins were expressed by protaryotic system and purified.The binding profile of NoV variants to saliva HBGAs was determined by the binding of P protein to a panel of saliva samples which HBGAs phynotypes had been identified previously.The binding profile of oligosaccharide was analyzed by the P protein binding to a panel of synthesized HBGAs oligosaccharide antigens.Results Sakai variant almost lost the binding capacity for saliva HBGAs,and other GⅡ.4 variants had the similar binding profile,which can bind to secretor saliva (A,B,AB and O secretor) and not bind to O non-secretor saliva.95/96US variant and 2006b variant had the highest binding capacities,followed by Camberwell variant and Hunter variant.The oligosaccharide binding profile was consistent with that of saliva HBGAs.Similar to the binding to saliva HBGAs,most variants bound to oligosaccharide secretor (Ley and H1) and did not bind to oligosaccharide secretor (Lea and Lex) except for Sakai variant.95/96US variant and 2006b variant had highest binding capacities,followed by Camberwell variant and Hunter variant,and Sakai variant lost the binding capacity.Conclusions Most GⅡ.4 NoV variants had the similar HBGAs binding profile,but their binding capacities were different,and the variant with high binding capacity caused the epidemic in broader region.