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PO_9稻瘟病原菌的菌丝悬浮液和孢子悬浮液,经无菌滤取和高压灭菌制备成稻瘟病菌粗毒素。采用浸根处理、心叶注射、叶肉注射、穗苞注射和叶面喷布5种方法,测定所制备的2种粗毒素对水稻的毒性。结果表明,本试验制备的粗毒素对水稻的根、叶、穗的幼嫩组织有较强的毒性;粗毒素经高温高压后,毒性略有减低;不同材料制备的粗毒素对水稻的毒性以菌丝悬浮液的较强。文中还讨论了稻瘟病菌毒素的毒性、制备及应用等。
The mycelial suspension and spore suspension of PO_9 were respectively prepared into the crude extracts of Magnaporthe grisea by aseptic filtration and autoclaving. Five methods of immersion root treatment, cardia injection, mesophyll injection, panicle injection and foliar spray were used to determine the toxicity of the two crude toxins to rice. The results showed that the crude toxin prepared in this experiment had strong toxicity to the young tissues of rice roots, leaves and spikes. The crude toxin was slightly reduced under high temperature and high pressure. The toxicity of crude toxins prepared from different materials to rice Mycelium suspension stronger. The paper also discussed the toxicity of Magnaporthe grisea toxin, preparation and application.