论文部分内容阅读
目的探讨ckit癌基因蛋白在肺神经内分泌癌中的表达及其临床病理意义。方法采用免疫组化SP法检测90例肺神经内分泌癌患者癌组织中ckit蛋白表达并分析其与各临床病理指标及预后的关系。结果ckit蛋白表达定位于癌细胞胞膜及胞质;ckit表达阳性率分别为类癌21.4%(3/14),不典型类癌90.0%(18/20),大细胞神经内分泌癌38.7%(12/31),小细胞癌76.0%(19/25),差异有统计学意义(P=0.000)。ckit表达癌在Ⅰ期为26.3%(5/19),Ⅱ期62.5%(15/24),Ⅲ期67.4%(29/43),Ⅳ期75.0%(3/4),差异有统计学意义(P=0.014)。ckit在患者性别中的分布为男性64.8%(46/71),女性31.6%(6/19),差异有统计学意义(P=0.017)。ckit表达癌在肿瘤直径大小的分布为<5cm者43.1%(22/51),≥5cm者76.9%(30/39),差异有统计学意义(P=0.002)。ckit表达在淋巴结转移患者为66.1%(47/71),无转移者26.3%(5/19),差异有统计学意义(P=0.003)。ckit阴性表达癌患者的中位生存时间为97个月,ckit阳性癌的患者中位生存时间为16个月,二者比较差异有统计学意义(LogRank=22.85,P=0.000)。ckit蛋白表达与患者肿瘤部位(P=1.000)、年龄(P=0.394)无关。结论ckit蛋白在肺神经内分泌癌的发生、演进过程中起着重要作用,并可作为分子指标监测患者预后。
Objective To investigate the expression of ckit oncogene protein in pulmonary neuroendocrine carcinoma and its clinicopathological significance. Methods Immunohistochemical SP method was used to detect the expression of ckit protein in 90 patients with pulmonary neuroendocrine carcinoma and its relationship with each clinicopathological parameters and prognosis. Results The expression of ckit protein was localized in the membrane and cytoplasm of cancer cells. The positive rates of ckit expression were 21.4% (3/14) for carcinoid, 90.0% (18/20) for atypical carcinoid, 38.7% for large cell neuroendocrine carcinoma 12/31), small cell carcinoma 76.0% (19/25), the difference was statistically significant (P = 0.000). The expression of ckit was 26.3% (5/19) in stage Ⅰ, 62.5% (15/24) in stage Ⅱ, 67.4% (29/43) in stage Ⅲ and 75.0% (3/4) in stage Ⅳ, the difference was statistically significant (P = 0.014). The distribution of ckit was 64.8% (46/71) in males and 31.6% in females (6/19), the difference was statistically significant (P = 0.017). The expression of ckit was 43.1% (22/51) in patients with tumor size of <5cm and 76.9% (30/39) in patients with ≥5cm, the difference was statistically significant (P = 0.002). The expression of ckit was 66.1% (47/71) in patients with lymph node metastasis and 26.3% (5/19) in patients without lymph node metastasis, the difference was statistically significant (P = 0.003). The median survival time of ckit-negative cancer patients was 97 months. The median survival time of ckit-positive cancer patients was 16 months. The difference was statistically significant (LogRank = 22.85, P = 0.000). The ckit protein expression was not related to the tumor location (P = 1.000), age (P = 0.394). Conclusion ckit protein plays an important role in the occurrence and evolution of pulmonary neuroendocrine carcinomas and can be used as a molecular marker to monitor the prognosis of patients.