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目的:探讨生态环境和遗传背景相同条件下8种不同变异类型甘草在基因表达水平上的差异,旨在揭示甘草种内变异的分子机制。方法:采用cDNA-AFLP、生物信息学等方法,对不同变异类型甘草在生长旺盛期根部组织特异表达的基因进行分析和功能预测;采用反相Northern杂交对特异表达的基因进行验证。结果:共获得14条差异表达基因片段,有6个基因序列编码的蛋白功能未知,其余的基因片段编码的蛋白参与了植物的抗菌,调节生物体内代谢,增加植物的抗热(高温)性,提高植物体固氮能力等生物过程。结论:首次克隆了不同变异类型甘草特异表达的基因,为筛选优良变异类型、品种选育、功能基因组的研究奠定基础。
OBJECTIVE: To investigate the difference in gene expression levels among eight different types of licorice under the same conditions of ecological environment and genetic background, and to reveal the molecular mechanism of intraspecific variation in licorice. Methods: The cDNAs of different genotypes of licorice at root exuberant period were analyzed and predicted by cDNA-AFLP and bioinformatics methods. The expression of specific genes was verified by reverse Northern blotting. Results: A total of 14 differentially expressed genes were obtained. The proteins encoded by 6 gene sequences were unknown. The proteins encoded by other genes were involved in the antibacterial activities of plants, regulating the metabolism in vivo, increasing the heat resistance (hyperthermia) Improve plant nitrogen fixation and other biological processes. CONCLUSION: The genes of different variation type licorice-specific expression were cloned for the first time, which laid the foundation for the screening of excellent variation types, breeding of varieties and functional genomics.