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对采自西藏拉萨、林芝和日喀则地区的 10属 18种豆科植物的 2 82份根瘤样品进行根瘤菌的分离纯化、菌株的回接和固氮酶活性的测定 ,结果表明 ,这些地区的豆科植物均可自然结瘤 ,其形态比较单一 ,主要为较规则的球形、柱状和掌状 ,颜色为褐红色、红色、粉红色和白色 ,球形根瘤的大小为 Φ0 .1～ 6 .0 m m,柱状根瘤的大小为 1～ 10 mm,92 .5 %的自然根瘤可测到固氮酶活性 ,其活性值为 0 .0 93～ 12 .40 3nmol/ (mg· h) ,可以看出 ,豆科植物根瘤的大小、颜色和固氮酶的活性与采集地点的生态环境相关。
Isolation and purification of rhizobia from 2282 nodular samples from 10 genera and 18 legumes collected from Lhasa, Nyingchi and Shigatse areas in Tibet, and the reconnection of strains and determination of nitrogenase activity showed that the number of legumes The plants are naturally nodulated, its morphology is relatively simple, mainly for the more regular spherical, columnar and palmate, the color is maroon, red, pink and white, spherical nodules size Φ0.1 ~ 6 .0 mm, The size of the columnar nodules was 1 ~ 10 mm, and 92.5% of the natural nodules detected the activity of nitrogenase, the activity value was 0 93 ~ 12. 40 3nmol / (mg · h), it can be seen that the legume The size, color and nitrogenase activity of plant nodules are related to the ecological environment at the collection site.