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目的将USPIO-PLL蛋白复合物标记人脐带间充质干细胞(MSCs)移植入小鼠LLC肺癌种植瘤模型,探索3.0T MR活体示踪技术。观察移植小鼠体内标记MSCs MR信号及肿瘤图像变化,探讨MSCs移植肺癌后的定向迁移、转化。方法培养MSCs,以多聚赖氨酸(PLL)为转染剂,用USPIO对MSCs-PLL进行磁性标记。将磁标记的MSCs通过鼠尾静脉移植入LLC肺癌种植瘤小鼠体内,分别于移植前、移植后1d,10d行MRI观察。结果 (1)USPIO-PLL可安全有效标记脐带间充质干细胞,标记率大于96%,细胞活性无影响。(2)移植后1d MRI可监测到MSCs到达病变区,10d到达病变区MSCs增多,MRI信号降低,差异有统计学意义(P<0.05)。建模成功后,MSCs组、NS组(生理盐水对照组)移植后1d,10d肿块体积组间差异均无统计学意义(P>0.05)。移植1d,10d抑瘤率均为正值。结论 MRI可以有效进行干细胞移植后的活体示踪,干细胞对LLC肺癌种植瘤有定向趋化作用,可以聚集至肿瘤区域,通过不同的抑瘤和促瘤生长发育机制相互交叉作用抑制肿瘤生长。
OBJECTIVE: To transplant the human umbilical cord-derived mesenchymal stem cells (MSCs) labeled with the USPIO-PLL protein complex into the LLC tumor model of lung cancer and to explore the 3.0T MR biopsy technique. To observe the changes of MR signals and tumor images of labeled MSCs in transplanted mice, and to explore the directional migration and transformation of MSCs after lung cancer transplanted. Methods MSCs were cultured and poly-L-lysine (PLL) was used as transfection reagent. MSCs-PLL was magnetically labeled with USPIO. Magnetically labeled MSCs were transplanted into the LLC lung cancer implanted tumor mice via the tail vein of the rats. MRI was performed before transplantation and 1 day and 10 days after transplantation, respectively. Results (1) USPIO-PLL can be used to label umbilical cord mesenchymal stem cells safely and effectively with the labeling efficiency greater than 96% and no effect on cell viability. (2) The MSCs reached the diseased area on the first day after transplantation, and the MSCs increased to the lesion site on the 10th day after the transplantation, and the MRI signal decreased. The difference was statistically significant (P <0.05). After successful modeling, there was no significant difference in volume between the MSCs group and the NS group (saline control group) on the 1st and 10th day after transplantation (P> 0.05). 1d, 10d tumor inhibition rate was positive. Conclusion MRI can effectively track the cells after stem cell transplantation. Stem cells can direct the chemotaxis of LLC implanted tumor to the tumor area and inhibit the tumor growth through different mechanisms of antitumor and tumor growth and development.