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采用Ｓｐｈｅｒｉ－５ＲＰ—１８不锈钢柱，以甲醇＋水（６４＋３６）为流动相和紫外检测器（２５４ｎｍ），对啶虫脒进行反相色谱分离和测定。其变异系数为０．０３％，标准偏差为０．０９７％，平均回收率为９９．９９％。
The acetamiprid was separated and determined by reverse phase chromatography using Spheri-5RP-18 stainless steel column with methanol + water (64 + 36) as mobile phase and UV detector (254nm). The coefficient of variation was 0.03%, the standard deviation was 0.097% and the average recovery was 99.99%.