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本文报道南京地区1984～1985年婴幼儿急性腹泻共收粪样402份,检测轮状病毒、肠道病毒和腺病毒。其中应用ELISA技术检测急性腹泻患儿粪样288例,检出率50.3%。对照组非腹泻幼儿粪样114例,检出率14.9%。经统计学处理P<0.01(x~2=40.38)两者有显著性差异。从阳性粪样中取10例作电镜观察为佐证,发现7例有轮状病毒颗粒。从急性患儿粪样选15例,在人胚肠原代细胞培养轮状病毒,发现2例传至第3代第3天呈现细胞病变
This article reports from 1984 to 1985 in Nanjing infant acute diarrhea were collected from a total of 402 samples of feces, detection of rotavirus, enterovirus and adenovirus. Among them, 288 cases of children with acute diarrhea were detected by ELISA, the detection rate was 50.3%. Control group of non-diarrhea 114 cases of fecal samples, the detection rate of 14.9%. The statistical analysis of P <0.01 (x ~ 2 = 40.38) showed significant difference between the two. Taking 10 cases of positive fecal samples for evidence of electron microscopy, found that 7 cases of rotavirus particles. Fifteen cases of acute fecal samples were selected and rotavirus was cultured in primary human embryo cells. Two cases were found to be cytopathic