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目的:探讨葛根素对去卵巢大鼠子宫雌激素受体(ER)α表达的影响。方法:大鼠去势后灌服不同剂量葛根素、倍美力溶液30d后,用酶联免疫吸附试验(ELISA)观察血清雌二醇(E2)水平,免疫组织化学法观察子宫ERα的表达。结果:大鼠去势后血清E2下降(P<0.01),给药30d后,给药各组血清E2水平及子宫ERα阳性表达明显上升,与模型组比较,有显著性差异(P<0.01)。结论:葛根素具有雌激素样作用,通过影响ERα的表达调节去卵巢大鼠生殖内分泌的功能。
Objective: To investigate the effect of puerarin on the expression of estrogen receptor (ER)α in ovariectomized rats. METHODS: After ovariectomized rats were given different doses of puerarin and premarin for 30 days, serum estradiol (E2) levels were observed by enzyme-linked immunosorbent assay (ELISA). The expression of ERα in the uterus was observed by immunohistochemistry. RESULTS: Serum E2 decreased after ovariectomized in rats (P<0.01). After 30 days of administration, serum E2 levels and uterine ERα positive expression were significantly increased in each group. Compared with the model group, there was a significant difference (P<0.01). . CONCLUSION: Puerarin has estrogen-like effects and regulates the reproductive endocrine function of ovariectomized rats by affecting the expression of ERα.