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目的:探讨生脉散对糖尿病心肌病小鼠心肌组织病理变化的影响。方法:将8周龄db/db小鼠随机分为模型组和生脉组,另设背景系小鼠C57BLKS/J为正常组。生脉组给予4.5g·kg-1·d-1生脉散灌胃,干预24周后检测小鼠空腹血糖、体质量、心脏质量、血流动力学指标,并以HE染色及透射电镜观察心肌组织形态变化、Masson染色观察心肌组织胶原纤维。结果:与正常组比较,模型组db/db小鼠空腹血糖、体质量、心脏质量及心肌胶原容积分数(CVF)显著升高(P<0.05),左室内压最大下降速率(-dp/dt)明显下降(P<0.05);生脉组db/db小鼠心脏质量与CVF较模型组明显减轻(P<0.05),而-dp/dt较模型组明显增加(P<0.05)。组织学检查显示,模型组db/db小鼠存在心肌细胞肥大、室壁增厚、间质纤维化、微血管病变及线粒体损伤,生脉组上述变化有一定程度的改善。结论:生脉散对db/db小鼠心肌损伤具有保护作用。
Objective: To investigate the effect of Shengmaisan on myocardial histopathological changes in diabetic cardiomyopathy mice. Methods: 8-week-old db / db mice were randomly divided into model group and Shengmai group, and C57BLKS / J mice in normal background were set as background. The rats in Shengmai group were given 4.5g · kg-1 · d-1 Shengmaisan intragastrically and the fasting blood glucose, body weight, heart weight and hemodynamics were measured 24 weeks after intervention. The cells were observed with HE staining and transmission electron microscope Myocardial morphological changes, Masson staining of myocardial collagen fibers. Results: Compared with the normal group, fasting blood glucose, body weight, heart mass and myocardial collagen volume fraction (CVF) in model group were significantly increased (P <0.05), and the maximum rate of decline in left ventricular pressure (-dp / dt ) (P <0.05). The heart mass and CVF of db / db mice in Shengmai group were significantly lower than those in model group (P <0.05), and -dp / dt was significantly increased compared with model group (P <0.05). Histological examination showed that there was myocardial hypertrophy, thickened walls, interstitial fibrosis, microvascular lesions and mitochondrial damage in model group db / db mice. The above changes in Shengmai group were improved to a certain degree. Conclusion: Shengmaosan has a protective effect on myocardial injury in db / db mice.