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目的克隆和分析7种斑叶兰属药用植物的ITS序列,为该属植物的分子鉴定提供依据。方法利用PCR法克隆ITS序列,在测序的基础上,借助生物信息学软件进行序列分析和系统发育分析。结果 7种斑叶兰属植物的ITS全长为700~702 bp,ITS1的长度为239~240 bp,ITS2为298~300 bp,而5.8 S的长度十分保守,均为162 bp;序列中检测到可变位点107个,其中信息位点34个。7种斑叶兰属植物的遗传距离为0.006~0.110,大花斑叶兰与高斑叶兰的遗传距离最大,亲缘关系最远,而斑叶兰和小斑叶兰的遗传距离最小,关系最近。结论克隆到7种斑叶兰属药用植物的ITS序列,它们信息位点丰富,这些位点可将7种植物完全区分。
Objective To clone and analyze the ITS sequences of seven medicinal plants of the genus Pleione to provide the basis for the molecular identification of this genus. Methods The ITS sequence was cloned by PCR and sequenced and phylogenetically analyzed by bioinformatics software on the basis of sequencing. Results The ITS sequences of seven species were 700 ~ 702 bp in length, 239 ~ 240 bp in ITS1 and 298 ~ 300 bp in ITS2, while the length of 5.8 S was very conservative To 107 variable sites, of which 34 information sites. The genetic distances among the seven species of Pleurotus were between 0.006 and 0.110. The genetic distance between P. grandiflora and P. tabulaeformis was the largest and the genetic distance was the most distant while the genetic distance between P. pleurotus and P. tabulaeformis was the smallest recent. CONCLUSIONS ITS sequences of seven medicinal plants of the genus Pleurotus were cloned and their informative sites were abundant. These seven sites could completely distinguish seven kinds of plants.