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目的 探讨 8 Hz 130 d B次声作用后代谢性谷氨酸受体 1α(m Glu R1α)在鼠脑各核团中表达的改变及其意义 .方法 SD大鼠 40只随机分为对照组及次声作用 1,7和 14次共 4组 ,次声作用组用 8Hz 130 d B的次声按规定次数作用 ,每次 2 h.多聚甲醛心脏灌注固定鼠脑 ,免疫细胞化学染色 ,光镜下观察 m Glu R1α在各核团表达改变 .结果 次声作用 1次组 ,大多数核团 m Glu R1α阳性神经元表达增多 ;至 7次组免疫反应阳性神经元数目增多显著 ,细胞淡染 ,中间低密度 ,类空泡样变 .核团内免疫阳性纤维同时增多 ,染色加深 ;14次组各核团阳性神经元减少至或低于正常水平 .结论 次声作用可引起脑内多数各核团神经元 m Glu R1α表达增高 ,提示 m Glu R1α是介导谷氨酸兴奋性毒性 ,引起神经元损伤的主要因素之一
Objective To investigate the significance of the expression of m Glu R1α in nuclei of rat brain nuclei after the infrasound of 8 Hz for 130 d and its significance.Methods 40 SD rats were randomly divided into control group The infrasound was performed in 4 groups of 1, 7, and 14 times. The infrasound group was infused with the infrasound of 8 Hz 130 d B for 2 h at each time, and paraformaldehyde was perfused into the brains of rats. Immunocytochemistry, light Microscopically, the expression of mRalR1alpha was changed in each of the nuclei.Results In the infrasound group, the expression of mRalR1alpha neurons in most of the nuclei increased, while in the seventh group, the number of immunoreactive neurons increased significantly, , The middle of the low-density, vacuolar-like changes.Nuclear immunoreactive fibers increased at the same time, deepened stained; 14 groups of nuclei positive neurons reduced to or below the normal level.Conclusion The infrasound can cause most Nuclear glutamate mRal R1α expression increased, suggesting that m Glu R1α is mediated by glutamate excitotoxicity, one of the main factors that cause neuronal damage