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目的:提供一种简易、经济、快速、便捷地诱发肥胖模型的方法。 方法:将雄性SD大鼠30只随机分为实验组和对照组,每组15只。实验组一次性腹腔注射维生素D300 U/g,对照组同时注射等量生理盐水。存活30 d后测Lee’s指数、脂肪系数、600倍光镜每视野脂肪细胞个数及血清瘦素水平。 结果:实验组Lee’s指数及脂肪系数(分别为313.7±6.9,0.26±0.05)明显高于对照组(分别为302.6±4.2,0.13±0.03)(t=5.352,9.455,P<0.05);实验组每视野脂肪细胞个数、血清瘦素水平[实验组:(31.2±17.6)μg/L,对照组:(7.5±6.3)μg/L]明显低于对照组(t=5.481,4.912,P<0.05)。 结论:一次性腹腔大剂量注射维生素D能方便快捷地诱发肥胖。
Objective: To provide a simple, economical, rapid and easy way to induce obesity models. Methods: Thirty male SD rats were randomly divided into experimental group and control group, with 15 rats in each group. Experimental group intraperitoneal injection of vitamin D300 U / g, while the control group injected with the same amount of saline. Survival 30 days after the Lee’s index, fat coefficient, 600 times the number of fat cells per field of vision and serum leptin levels. Results: The Lee’s index and fat coefficient (313.7 ± 6.9 and 0.26 ± 0.05 respectively) in the experimental group were significantly higher than those in the control group (302.6 ± 4.2 and 0.13 ± 0.03 respectively) (t = 5.352 and 9.455, P <0.05) The number of adipocytes per field, serum leptin level (31.2 ± 17.6 μg / L in the experimental group and 7.5 ± 6.3 μg / L in the control group) were significantly lower than those in the control group (t = 5.481, 4.912, P < 0.05). Conclusion: One-time high-dose intraperitoneal injection of vitamin D can induce obesity easily and quickly.