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目的观察细胞因子参与下脐血干细胞体外扩增效果。方法用含15%胎牛血清改良细胞培养液,加入不同的细胞因子,设置1个对照组和3个实验组,分别于培养第0d、3d、7d、14d取培养液,台盼蓝染色计数单个核细胞的总数、流式细胞仪分析CD34+细胞含量。结果与对照组相比,实验组单个核细胞的数量和CD34+细胞的百分比均有显著增加,尤其以Ⅲ组(IL-3,6,2,4)第7d的扩增为最高:单个核细胞总数达(17.11±6.12)×109个/L,CD34+细胞比率达(9.92±2.56)%。结论细胞因子可以提高脐血单个核细胞和CD34+细胞的扩增效率,SCF+IL-3+6+2+4的组合在第7d时达到了最佳的扩增。
Objective To observe the effects of cytokines on the expansion of cord blood stem cells in vitro. Methods The cell culture medium containing 15% fetal bovine serum was added with different cytokines. One control group and three experimental groups were set up. The culture medium was taken on the 0d, 3d, 7d and 14d respectively. The trypan blue staining The total number of mononuclear cells, flow cytometry CD34 + cell content. Results Compared with the control group, the number of mononuclear cells and the percentage of CD34 + cells in experimental group were significantly increased, especially in group Ⅲ (IL-3, 6, 2, and 4) The total number was (17.11 ± 6.12) × 109 cells / L, and the percentage of CD34 + cells was (9.92 ± 2.56)%. Conclusions Cytokines can increase the efficiency of cord blood mononuclear cells and CD34 + cells. The combination of SCF + IL-3 + 6 + 2 + 4 achieves the best amplification on the 7th day.