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目的:探讨单纯疱疹病毒Ⅰ型(HSV-Ⅰ)F株初次感染后在脑内是否形成潜伏感染。方法:用角膜划痕法给Balb/c小鼠接种单纯疱疹病毒Ⅰ型F株,6周后用免疫组化法检测脑组织不同部位及三叉神经节HSV-1抗原的表达,聚合酶链反应(PCR)方法分别检测小鼠脑的颞叶、脑干、小脑及三叉神经节疱疹病毒DNA片段。结果:病毒接种6周后,小鼠三叉神经节、脑组织的不同部位未检测到HSV-1抗原的表达;三叉神经节、脑的颞叶、脑干和小脑均检测到了疱疹病毒DNA片段。结论:单纯疱疹病毒除了可以在三叉神经节建立潜伏感染外,脑组织也是其建立潜伏感染的部位之一。
Objective: To investigate whether latent infection of herpes simplex virus type 1 (HSV-Ⅰ) strain F in the brain after primary infection was established. Methods: Balb / c mice were inoculated with herpes simplex virus type Ⅰ strain F by corneal scratching method. Six weeks later, the expression of HSV-1 antigen in different parts of the brain tissue and trigeminal ganglion was detected by immunohistochemistry. The expression of HSV- (PCR) were used to detect the temporal lobe, brainstem, cerebellum and trigeminal herpesvirus DNA fragments in the brain of mice. Results: Six weeks after virus inoculation, HSV-1 antigen was not detected in the trigeminal ganglia and brain tissues of mice. Herpesvirus DNA fragments were detected in the trigeminal ganglia, temporal lobe, brainstem and cerebellum. Conclusion: Herpes simplex virus in addition to the establishment of latent infection in the trigeminal ganglion, brain tissue is also one of the parts of the establishment of latent infection.