论文部分内容阅读
目的化学物质的毒性作用常因其在体内代谢而变化。为探讨酒精在代谢条件下的发育毒性，本研究应用植入后全胚胎培养技术研究了酒精对胚胎发育的影响。方法：孕９５天大鼠胚胎于体外条件下移植分离培养在含４０、１００、２０、４０ｍｇ／ｄｌ内含Ｓ９的即可离心大鼠血清培养基中连续培养４８小时，观察酒精对胚胎生长发育和器官形态分化的影响。结果：４０ｍｇ／ｄｌ＋Ｓ９组与对照组各指标无显著性差异，１００ｍｇ／ｄｌ＋Ｓ９组则导致胚胎的体长、ＤＮＡ、蛋白质含量及部分器官形态评分呈剂量相关的下降，但前脑、中脑评分并无影响。随染毒剂量增加，胚胎的绝大多数指标显著降低，胚胎致畸率和死亡率相应增高。结论：酒精在Ｓ９作用下转化为乙醛后，毒性作用的靶器官发生改变，表现为肢芽、心脏等器官发育异常为主，代谢产物乙醛也有一定的致畸作用。
The toxic effects of the target chemical often change due to its metabolism in the body. In order to investigate the developmental toxicity of alcohol under metabolic conditions, we investigated the effect of alcohol on embryonic development using post-implantation whole-embryo culture. METHODS: Pregnant 9 5 days old embryos were transplanted in vitro and cultured in vitro for 40 hours in a centrifuge rat serum containing 40, 100, 20 and 40 mg / dl of S9 for 48 hours. The effect of alcohol on embryos Growth and development and organ morphological differentiation. Results: There were no significant differences in the indexes of 40mg / dl + S9 group and the control group. The body weight, DNA, protein content and morphological score of some organs in 100mg / dl + S9 group decreased in a dose dependent manner. no effect. With the increase of exposure dose, most of the embryo’s indexes decreased significantly, and the embryo’s teratogenic rate and mortality increased accordingly. CONCLUSION: Alcohol is the target organ of toxic effect after S9 is converted to acetaldehyde. The main organ abnormalities such as limb bud and heart are the main abnormalities. The metabolite acetaldehyde also has some teratogenic effects.